ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Single-cell transcriptomics for microbial eukaryotes

One of the greatest hindrances to a comprehensive understanding of microbial genomics, cell biology, ecology, and evolution is that most microbial life is not in culture. Solutions to this problem have mainly focused on whole-community surveys like metagenomics, but these analyses inevitably loose information and present particular challenges for eukaryotes, which are relatively rare and posses...

cgCorrect: a method to correct for confounding cell-cell variation due to cell growth in single-cell transcriptomics.

Accessing gene expression at a single-cell level has unraveled often large heterogeneity among seemingly homogeneous cells, which remains obscured when using traditional population-based approaches. The computational analysis of single-cell transcriptomics data, however, still imposes unresolved challenges with respect to normalization, visualization and modeling the data. One such issue is dif...

Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics

Single-cell transcriptomics allows researchers to investigate complex communities of heterogeneous cells. These methods can be applied to stem cells and their descendants in order to chart the progression from multipotent progenitors to fully differentiated cells. While a number of statistical and computational methods have been proposed for analyzing cell lineages, the problem of accurately ch...

Evidence of Double Dissociation in Auditory Agnosia: Implications for Rehabilition

Dissociation Constant in Nitrogen Fixation by Azotobacter

6 Dodge originally used "A" and "a" to designate the two sex types in Neurospora crassa. Lindegren later substituted the symbols "+" and "-" for these. Because of the desirability of using the symbol + for the normal allele of a gene and also to designate a positive experimental result, we prefer Dodge's original designations. 4 Neither conversion of pimelic acid to p-aminobenzoic acid by the n...